Coding

Part:BBa_K4147006

Designed by: Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-10-08)
Revision as of 04:09, 10 October 2022 by Ana Belem (Talk | contribs)


Disulfide interchange protein DsbA from Pseudomonas aeruginosa

The DsbA enzyme from P. aeruginosa is one of the most oxidizing proteins yet characterized [1]. This protein introduces disulfide bonds into proteins secreted to the periplasm. It was later optimized for E. coli strains.

Usage and Biology

Fusing a recombinant protein's N-terminus to a signal peptide is one way to increase the solubility and functionality of the protein in E. coli. This can cause the heterologous protein to be exported from the cytoplasm into the periplasm or the culture media via the type II secretion system [1]. One of the recognized proteins that oxidizes the most is DsbA. Although DsbA homologs from other bacteria, such as Pseudomonas aeruginosa, are even more oxidizing than E. coli DsbA, which has a redox potential of -119 mV (-95 mV). Numerous secreted polypeptides, including virulence factors, are folded oxidatively by DsbA. About 300 proteins in E. coli, or 40% of the periplasmic proteome, have an even number of cysteine residues and may therefore be folded by DsbA [2].

Adding proteins to DsbA peptides can improve the target molecule's performance and solubility while also drastically reducing the creation of inclusion bodies [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 403
  • 1000
    COMPATIBLE WITH RFC[1000]


REFERENCES

[1] Zhang, W., Lu, J., Zhang, S., Liu, L., Pang, X., & Lv, J. (2018). Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study. Microbial Cell Factories, 17(1). doi:10.1186/s12934-018-0894-y

[2] Shouldice, S. R., Heras, B., Jarrott, R., Sharma, P., Scanlon, M. J., & Martin, J. L. (2010). Characterization of the DsbA Oxidative Folding Catalyst from Pseudomonas aeruginosa Reveals a Highly Oxidizing Protein that Binds Small Molecules. Antioxidants & Redox Signaling, 12(8), 921–931. doi:10.1089/ars.2009.2736

[edit]
Categories
Parameters
None