Composite

Part:BBa_K4452018

Designed by: iGEM22_Hopkins   Group: iGEM22_Hopkins   (2022-09-30)
Revision as of 04:08, 10 October 2022 by KalenClifton (Talk | contribs)

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nptII antibiotic resistance for expression in plants

For selection of positively transformed seedling on agar plates, nptII confers resistance to neomycin/kanamycin. This antibiotic resistance gene is expressed with the constitutive CMV35S plant specific promoter, a 5’ UTR from the AtRbcS2B gene, and a 3’UTR plant terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1472
    Illegal BsaI.rc site found at 1751


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Parameters
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