Composite

Part:BBa_K4260007

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-09)
Revision as of 03:47, 10 October 2022 by Registry (Talk | contribs)

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Isoeugenol Monooxygenase, normal coding sequence: promoter, RBS, pelB, Iso and terminator

BBa_K4260006 is a composite part that aims to work as a selection marker for the replacement of antibiotic-based selection markers. Isoeugenol monooxygenase (coded by gene Iso) is an enzyme present in Pseudomonas bacteria, that converts isoeugenol to vanillin directly. Isoeugenol is an aromatic compound found in clover and cinnamon essential oils, that has proved to have an inhibitory effect on different microorganisms; however, this genetic construct was designed for its expression in Escherichia coli. Its antimicrobial activity resides in altering the integrity of the inner membrane of the bacteria, leading to fluid spillage and cell death. Therefore, by directing the enzyme to the periplasm of the bacteria, only transformed cells could survive when exposed to isoeugenol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 101
    Illegal NgoMIV site found at 809
    Illegal NgoMIV site found at 1417
    Illegal AgeI site found at 633
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729


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Categories
Parameters
None