Part:BBa_K4137003
Chi18h8-YebF Fusion Protein
This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality.
Construct Design
During the gBlocks fragment synthesis stage of our construct design, we were forced to split Chi18h8 chitinase along the restriction sites ClaI due to errors in IDT gblock fragment synthesis, in which the first half would have cut sites at EcoRI and ClaI and the second half would have cut sites at ClaI and SpeI. We first ligated the first half of chitinase onto our pSB1C3 backbone before attaching the second half. The full Chi18h8 construct would have EcoRI and SpeI cut sites.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1492
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1005
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 552
- 1000COMPATIBLE WITH RFC[1000]
| None |