Coding

Part:BBa_K4137003

Designed by: YA-JHIH KO   Group: iGEM22_GEMS_Taiwan   (2022-08-20)
Revision as of 03:17, 10 October 2022 by Andreahuang (Talk | contribs)


Chi18h8-YebF Fusion Protein

This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality.

Construct Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1492
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1005
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 552
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None