Generator

Part:BBa_K4325015

Designed by: Laiyi Teng   Group: iGEM22_SZPT-CHINA   (2022-09-30)
Revision as of 03:04, 10 October 2022 by Taaa (Talk | contribs)


J23102-RBS003422-gshF-T0

Description

The composite part is a generator consisting of a J23102 promoter and gshF.

Sequence and Features


2022 SZPT-China

Biology

This composite part is a generator consisting of a J23102 (BBa_J23102) promoter and gshF (BBa_K4325003).

Usage

The J23102 promoter and gshF were connected and inserted into the pSEVA331 expression vector so that gshF expressed the novel bifunctional enzyme GshF, which directly catalyze the synthesis of glutathione by the three amino acids of Cys, Glu and Gly.

Characterization

1.Verification of gshF in E. coli

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