Part:BBa_K4260012
Isoeugenol Monooxygenase, inducible coding sequence: Trp term, IemR, promoter, pelB, Iso & rrnBT1 te
BBa_K4260006 is a composite part that aims to work as a selection marker for the replacement of antibiotic-based selection markers. Isoeugenol monooxygenase (coded by gene Iso) is an enzyme present in Pseudomonas bacteria, that converts isoeugenol to vanillin directly. Its transcription is regulated by the expression of its transcriptional activator (IemR), as well as the presence of isoeugenol. Since the substrate of the enzyme is isoeugenol and the inducer of its promoter is also isoeugenol, it can be considered an auto-inducible transcription regulation system.
Isoeugenol is an aromatic compound found in clover and cinnamon essential oils, that has proved to have an inhibitory effect on different microorganisms; however, this genetic construct was designed for its expression in Escherichia coli. Its antimicrobial activity resides in altering the integrity of the inner membrane of the bacteria, leading to fluid spillage and cell death. Therefore, by directing the enzyme to the periplasm of the bacteria, only transformed cells could survive when exposed to isoeugenol.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 439
Illegal XhoI site found at 267
Illegal XhoI site found at 294 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1187
Illegal NgoMIV site found at 1895
Illegal NgoMIV site found at 2503
Illegal AgeI site found at 1719 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1123
Illegal BsaI.rc site found at 1815
| None |