Generator

Part:BBa_K215000

Designed by: Chris Eiben   Group: iGEM09_Washington   (2009-09-30)
Revision as of 03:08, 1 October 2009 by Siegeljb (Talk | contribs)

R0011+B0034, strong IPTG-inducible promoter with strong RBS.

This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence. This part is in pSB1A3, which has a built in terminator at the end. This allows for a protein coding sequence to be inserted into this vector and be immediately ready for expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmidbackbone/expression/inducible
Parameters
n/aR0011+B0034, strong IPTG-inducible promoter with strong RBS.