Composite

Part:BBa_K4147011

Designed by: Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-10-08)
Revision as of 23:50, 9 October 2022 by Ana Belem (Talk | contribs)


mCherry (RFP) construct with lacI regulated, lambda pL hybrid promoter for bacterial expression

This mCherry reporter is one of several "second-generation" monomeric fluorescent proteins. The proposed construct for its expression it's conformed by a LacI regulated, lambda pL hybrid promoter (BBa_R0011), an RBS (BBa_B0032) and a double terminator (BBa_B0015). Lastly this construct was optimized for its production on E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization of RFP construct with lacI regulated, lambda pL hybrid promoter for bacterial expression

MCherry-Tec-Chihuahua-hibrido.png
Figure 1. SnapGeneÂ®ï¸ map of BioBrick BBa_K4147011.


[edit]
Categories
Parameters
None