Part:BBa_K4447005
Bacterial Expression System for the Detection of Erythromycin based on FRET
Expression system based on FRET biosensor for the detection of erythromycin. This system is suitable for cloning in pSB1C3 as the plasmid backbome, which propagates the BioBrick part and is widely used by other iGEM teams.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 3157 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3812
Illegal SapI site found at 961
Contents
Usage and Biology
The usage of genetic constructs that are designed to produce a protein, either inside or outside a cell, is important in several ways, such as increasing the expression rate. With this BioBrick, we are generating a bacterial expression platform that is capable of generating several copies of our protein of interest, EryK, coupled with the FRET system for the detection of erythromycin.
Our expression platform is composed of the following elements: an inducible promoter, which is regulated in response to specific stimuli; a ribosome-binding site to bind the ribosome for the initiation of translation; and a transcription terminator<b> that mediates the release of the transcript RNA from the translational complex.
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