Coding

Part:BBa_K4447005

Designed by: Rafael Garcia Gomez   Group: iGEM22_TecMonterrey_GDL   (2022-09-30)
Revision as of 22:45, 9 October 2022 by Rafa1470 (Talk | contribs)


Bacterial Expression System for the Detection of Erythromycin based on FRET

Expression system based on FRET biosensor for the detection of erythromycin. This system is suitable for cloning in pSB1C3 as the plasmid backbome, which propagates the BioBrick part and is widely used by other iGEM teams.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3812
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None