Regulatory

Part:BBa_K3748011

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)
Revision as of 22:13, 9 October 2022 by Kiselev sl (Talk | contribs)


pPAB1

S.cerevisiae medium strenght promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Estonia_TUIT characterization of BBa_K3748015 (pPAB1)

Constitutive pPAB1 promoter (Michael E. Lee et. al, 2015), controls a gene encoding the Pab1<i> protein. This protein binds Poly(A) and is involved in the control of poly(A) tail length on nuclear mRNA transcripts (N Amrani et. al, 1997).

<i>pPAB1 promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, pPAB1 showed a 19-fold increase in fluorescence intensity (figure 1).

Figure 1: Graph depicting median fluorescence intensity of pPAB1 and control strain

References:

  • A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015
  • Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro, N Amrani, M Minet, M Le Gouar, F Lacroute, F Wyers, 1997
[edit]
Categories
//promoter
Parameters
None