Terminator

Part:BBa_K4260008

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-09)
Revision as of 22:03, 9 October 2022 by Registry (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)


Terminator.png

Tryptophan operon terminator

  • This part is a 20 bp-long terminator, from the Trp operon, that forms a stem-loop structure. The tryptophan operator provides transcription efficiency as a terminator design, promoting a rho-independent or intrinsic termination. This means that the termination process carried out by the Trp terminator does not depend on the presence of termination factors [1]. Moreover, it shares several features with other known sites of transcription termination of E. Coli. The tryptophan terminator was designed with scientific literature and iGEM team's research, in this way it was used for the transcription termination of a gene of another part (to know more, please visit BBa_K4260006), that was tested in E.coli DH5α and BL21 strains with experimental procedures.

Secondary Structure

File:Mfold-K4260008-1.png


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
[edit]
Categories
Parameters
None