Composite

Part:BBa_K4147004

Designed by: César Ibrahym Rodríguez Fernández   Group: iGEM22_Tec-Chihuahua   (2022-09-30)
Revision as of 21:44, 9 October 2022 by Ana Belem (Talk | contribs)


DrsB1 Construct with LacI Promoter

Antimicrobial protein from the arboreal tree frog Phyllomedusa bicolor, dermaseptin-b1 gene (AKA MsrA2) with added parts as a single sequence to promote expression, being optimized for E. coli. Other than the original sequence for MsrA2 (BBa_K2577001), we added a LacI promoter (BBa_R0010), an RBS (BBa_B0032) and a double terminator (BBa_B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization of PSB1C3-DrsB1-Existente-CC27 (1) Map.png

PSB1C3-DrsB1-Existente-CC27 (1) Map.png
Figure 1. SnapGeneÂ®ï¸ map of BioBrick BBa_K4147004.


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Categories
Parameters
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