Coding

Part:BBa_K4461012

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-09)
Revision as of 21:34, 9 October 2022 by Registry (Talk | contribs)

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mCerulean+tag fusion protein

We synthesized a sequence of tag (BBa_K1051207) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert tag into our construct (BBa_K4461011) by restriction enzyme digestion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None