Composite

Part:BBa_K4129123:Design

Designed by: Magnus Haahr   Group: iGEM22_DTU-Denmark   (2022-10-09)
Revision as of 21:19, 9 October 2022 by Registry (Talk | contribs)

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The fungal synthetic expression system with FunsTF05 and 6xLexO-Pmin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BglII site found at 3258
    Illegal BamHI site found at 2920
    Illegal BamHI site found at 5785
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3113
    Illegal XhoI site found at 3550
    Illegal XhoI site found at 4455
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 6421
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 6135
    Illegal SapI site found at 323


Design Notes

Source

This part was assembled using USER cloning of parts that were either amplied from in-house plasmids or synthesied as gene block.

References