Part:BBa_K4286203
PCaMV-prb1-6xHis-TNOS
The PRB1 gene encodes a serine protease which plays an important role in the destruction of the plant pathogens. It is widely found in various trichoderma species which attack a large variety of phytopathogenic fungi responsible for major crop diseases. During the interaction, Trichoderma penetrates into the host mycelium, by partial degradation of its cell wall. It appears that the main mechanism involved in the antagonism to pathogenic fungi by Trichoderma is the release of lytic enzymes including PRB1. PRB1 production in Trichoderma is controlled by two mechanisms: it is induced by the presence of a phytopathogenic fungus, or its cell walls, and repressed by glucose. Evidence suggests that over-expression of the proteinase-encoding gene prb1 improved biocontrol activity of Trichoderma. To prevent Rice Sheath Blight, we choose Trichoderma atroviride(T.atroviride) as our chassis to overexpress prb 1. The gene circuit of PRB1 is consist of CamV 35s promoter, prb1 gene, 6xHis affinity tag, NOS terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1946
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1046
Illegal NgoMIV site found at 1091
Illegal NgoMIV site found at 1385
Illegal NgoMIV site found at 1499
Illegal AgeI site found at 2015 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 66
Illegal BsaI site found at 1232
Illegal BsaI.rc site found at 1109
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