Composite

Part:BBa_K4147008

Designed by: Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-10-08)
Revision as of 20:34, 9 October 2022 by Ana Belem (Talk | contribs)


PelB-(CBD)2-DrsB1 Construct with DsbA

This part contains the (CBD)2-DrsB1 expression construct (BBa_K4147001) with co-expression of DsbA (BBa_K4147006) for disulfide bond formation and proper folding of the osmotin protein.

Usage and Biology

The disulfide bonds are essential for structure stabilization and biological functions of these peptides [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1184
    Illegal NgoMIV site found at 1368
    Illegal AgeI site found at 392
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization of PelB-(CBD)2-DrsB1 Construct with DsbA

PelB-(CBD)2-DrsB1 Construct with DsbA Map.png
Figure 1. SnapGeneÂ®ï¸ map of BioBrick BBa_K4147008.

REFERENCES

[1] Zhang QY, Yan ZB, Meng YM, et al. Antimicrobial peptides: mechanism of action, activity and clinical potential. Mil Med Res. 2021;8(1):48. Published 2021 Sep 9. doi:10.1186/s40779-021-00343-2


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