Regulatory

Part:BBa_K4129115:Design

Designed by: Magnus Haahr   Group: iGEM22_DTU-Denmark   (2022-10-09)
Revision as of 19:49, 9 October 2022 by Registry (Talk | contribs)

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Minimal gpdA promoter fused with six LexO bindings sites (6xLexO-Pmin)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part was assembled using USER cloning. The minimal gpdA promoter was amplifed from an in-house plasmid carring the TetOn system. The six lexO binding sites, 6xLexO, were amplifed from a in-house plasmid granted to us from Michael Krogh Jensen from The Novo Nordisk Foundation Center for Biosustainability.

References