Protein_Domain

Part:BBa_K4428001:Design

Designed by: Dhwani Teckchandani   Group: iGEM22_IIT-Delhi   (2022-10-09)
Revision as of 18:58, 9 October 2022 by Dhwaniteck (Talk | contribs) (→‎References)


PbrR MBD - Lead-binding domain of PbrR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 20


Design Notes

Codon optimised for the chassis E. coli by changing the 150th nucleotide from T to C


Source

Truncated part of PbrR

References

Hui, CY., Guo, Y., Yang, XQ. et al. Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption. Biotechnol Lett 40, 837–845 (2018). https://doi.org/10.1007/s10529-018-2533-4