Protein_Domain

Part:BBa_K4428001

Designed by: Dhwani Teckchandani   Group: iGEM22_IIT-Delhi   (2022-10-09)
Revision as of 18:31, 9 October 2022 by Registry (Talk | contribs)

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PbrR MBD - Lead-binding domain of PbrR

PbrR was modified by truncating the N-terminal DNA Binding Domain and C-terminal redundant amino acid residues to obtain lead domain maintaining its Pb2+ binding property. The MBD (metal binding domain) being smaller in size leads to increased display and expression than the full-length PbrR-displayed cells.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 20


[edit]
Categories
Parameters
None