Coding
Part:BBa_K209400:Design
Designed by: Benjamin Rhau Group: iGEM09_UCSF (2009-09-29)
AarI B-C part, hM4D
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 184
Illegal PstI site found at 979 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1215
Illegal PstI site found at 184
Illegal PstI site found at 979 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 184
Illegal PstI site found at 979 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 184
Illegal PstI site found at 979
Illegal NgoMIV site found at 1086 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Cloned into pDONR221.
Source
Plasmid obtained from Bryan Roth lab, UNC.