Coding

Part:BBa_K4229010

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 17:29, 9 October 2022 by NikitaEdel (Talk | contribs)


SpyCatcher


The SpyCatcher and SpyTag is a system, which was developed from from a surface protein from Streptococcus pyogenes, which makes it possible to link Proteins with each other. In our project, we linked the T1 protein of the wiffelballs with the spyTag to XiaI, of our indigo-pathway. XiaI is fused with the SpyCatcher as you can see in BioBrick BBa_K4229012.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None