Part:BBa_K4192130
Plac-RBS-yf1-RBS-fixJ-Pfixk2-RBS-mcherry
The gene yf1, fixJ and PfixK2 make up the system of blue light regulation. Under dark conditions, phosphate groups are transferred from Yf1 protein to FixJ protein, and the phosphorylated FixJ protein activates the PfixK2 promoter, which in turn activates downstream gene expression. Under light induction, the phosphorylation of Fixj protein was blocked and the expression of genes regulated by the PfixK2 promoter was suppressed.The mCherry protein emits red fluorescence to characterize the efficiency of control elements in regulating gene expression.
Usage
Light control system can regulate gene expression by light intensity. Mcherry has an excitation wavelength of 580nm and an emission wavelength of 610nm. Quantitative detection of mcherry can be achieved by detecting the optical signal at 610nm. This composite part was constructed to test the ability of the original light control system to regulate gene expression compared with the light control system containing yf1 mutation(BBa_K4192132).
Biology
Fig.2 Regulatory mechanism of YF1 construction[1]</b>
Sequence and Features
Illegal SpeI site found at 1361
Illegal SpeI site found at 2257
Illegal SpeI site found at 1361
Illegal SpeI site found at 2257
Illegal SpeI site found at 1361
Illegal SpeI site found at 2257
Illegal SpeI site found at 1361
Illegal SpeI site found at 2257
Illegal NgoMIV site found at 764
Illegal NgoMIV site found at 836
Illegal NgoMIV site found at 926
Illegal NgoMIV site found at 944
Illegal NgoMIV site found at 1450
Illegal NgoMIV site found at 1743
Illegal NgoMIV site found at 1837
Illegal AgeI site found at 478
Illegal AgeI site found at 1618
Illegal BsaI.rc site found at 377
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