Part:BBa_K200007

Cellulase

Overview

This is the coding sequence for [http://en.wikipedia.org/wiki/Cellulase cellulase] production in E.coli. It codes for endo-b-1,4-glucanase E (CelE) cellulase, which is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.1

More Details

Cellulase mainly catalyses the reactions that changes crystalline cellulose to cellobiose2 and then finally to glucose. It also catalyses, to a small extent, the break down of carboxymethyl cellulose. This cellulase is protease resistant.

The gene was used as part of the Imperial iGEM 2009 The E.ncapsulator project as one of the showcase proteins of the protein production mechanism of the project.

Usage and Biology

It can be used in gram positive bacteria.

The enzyme has a C-end cellulosome-binding domain (CBD), which is used to deliver its resident catalytic domain to the cellulosome. The cellulosome is multi-subunit complex that is involved in the hydrolysis of crystalline cellulose. [http://www.springerlink.com/content/lp8g657n13g76187/fulltext.pdf [3]]

In Clostridum, CelE activity activates other cellulosomal enzymes synergistically.2

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 199
  Illegal PstI site found at 700
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 700
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 287
  Illegal BamHI site found at 814
  Illegal XhoI site found at 524
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 199
  Illegal PstI site found at 700
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 199
  Illegal PstI site found at 700
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI] <biblio>

1. 1 pmid=9055408
2. 2 pmid=10714996

</biblio>