Composite

Part:BBa_K4343073

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 14:41, 9 October 2022 by Registry (Talk | contribs)

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pUC-HUH-FmDes12

FmDes12 gene encodes a ∆-12 desaturase from Fusarium moniliform, which can catalyze C18:1 to C18:2. This gene is expressed in Y. lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1920
    Illegal XhoI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2108
    Illegal NgoMIV site found at 5373
    Illegal AgeI site found at 97
    Illegal AgeI site found at 458
    Illegal AgeI site found at 3161
    Illegal AgeI site found at 3522
    Illegal AgeI site found at 4664
    Illegal AgeI site found at 6120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 638
    Illegal BsaI site found at 3702
    Illegal BsaI site found at 4239
    Illegal BsaI site found at 4667
    Illegal BsaI site found at 6123
    Illegal BsaI.rc site found at 4661
    Illegal BsaI.rc site found at 6117
    Illegal BsaI.rc site found at 6617
    Illegal BsaI.rc site found at 6627
    Illegal SapI.rc site found at 363
    Illegal SapI.rc site found at 3427


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Categories
Parameters
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