Part:BBa_K239001:Design

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

A third envelope stress signal transduction pathway in Escherichia coli. Robert G. Raffa and Tracy L. Raivio* Molecular Microbiology (2002) 45(6), 1599–1611

Genome-Wide Analyses of Escherichia coli Gene Expression Responsive to the BaeSR Two-Component Regulatory System Kunihiko Nishino, Takeshi Honda, and Akihito Yamaguchi J Bacteriol. 2005 March; 187(5): 1763–1772. doi: 10.1128/JB.187.5.1763-1772.2005. PMCID: PMC1063996

The BaeSR two-component regulatory system mediates resistance to condensed tannins in Escherichia coli. Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008 Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.

Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651. PMCID: PMC2731931

Signal Detection and Target Gene Induction by the CpxRA Two-Component System Patricia A. DiGiuseppe and Thomas J. Silhavy J Bacteriol. 2003 April; 185(8): 2432–2440. doi: 10.1128/JB.185.8.2432-2440.2003. PMCID: PMC152615