Composite

Part:BBa_K4286100

Designed by: Minxi Zeng，Yinfeng Wu   Group: iGEM22_SZU-China   (2022-09-17)
Revision as of 14:12, 9 October 2022 by ZengMinxi (Talk | contribs)


Effector device in timed suicide switch

2022 SZU-China plans to design a timed suicide switch mediated by a classical synthetic gene oscillator. The core of the effector is the MazEF toxin-antitoxin system, in which the antitoxin mazE is controlled by the Promoter tetR , and the antitoxin mazF is controlled by the constitutive promoter PJ23110. The MazEF system is an important component of the subtilisin-antitoxin system, which leads to programmed cell death. MazF is an mRNA endonuclease that specifically cleaves a five-base UACAU sequence on RNA. In the presence of MazE, MazF has a higher affinity for MazE than its RNA substrate. MazE binds to MazF to occupy its active site, rendering it unable to bind or cleave the mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 481
    Illegal NheI site found at 504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None