Composite

Part:BBa_K4343076

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 14:11, 9 October 2022 by Registry (Talk | contribs)

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pUC-HUH-PaDes17

The PaDes17 gene encodes a ∆-17 elongase from Pythium aphanidermatum that catalyzes the conversion of arachidonic acid (ARA; C20:4 n-6) to Eicosapentaenoic acid (EPA), an ω 3 polyunsaturated fatty acid (PUFA).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5099
    Illegal XhoI site found at 1920
    Illegal XhoI site found at 1953
    Illegal XhoI site found at 4841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2108
    Illegal AgeI site found at 97
    Illegal AgeI site found at 458
    Illegal AgeI site found at 3161
    Illegal AgeI site found at 3522
    Illegal AgeI site found at 4664
    Illegal AgeI site found at 5766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 638
    Illegal BsaI site found at 3702
    Illegal BsaI site found at 4239
    Illegal BsaI site found at 4667
    Illegal BsaI site found at 5769
    Illegal BsaI.rc site found at 4661
    Illegal BsaI.rc site found at 5763
    Illegal BsaI.rc site found at 6033
    Illegal SapI.rc site found at 363
    Illegal SapI.rc site found at 3427


[edit]
Categories
Parameters
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