Part:BBa_K4165053

HtrA1 Switch 33

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_K4165063), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_K4165063), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721).

Usage and Biology

This part performs a multifunction role in our system, the Plug-Sink system. The H1A (BBa_K4165000) peptide directs the whole peptide to bind to the PDZ HtrA1 protein (BBa_K4165004). The second part is the binding peptide which directs the whole peptide-HtrA1 complex to the tau (BBa_K4165009) and amyloid (BBa_K4165005) aggregates. The last part makes the whole system switch on or off based on the presence of the target protein, thus part is the WAP inhibitor (BBa_K4165008).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 589
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 572
  Illegal AgeI site found at 308
  
• 1000
  COMPATIBLE WITH RFC[1000]

Modelling

PDB Structure:

                   Figure 1. The 3D structure of switch 33 modelled by TRrosetta.