Composite

Part:BBa_K4343092

Designed by: huanyu li   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 14:09, 9 October 2022 by Registry (Talk | contribs)

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pUC-HUH-Scp2-EhElo9(PFABin)

EhElo9 encodes ∆-9 elongase from Emiliania huxleyi which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1431
    Illegal XhoI site found at 3465
    Illegal XhoI site found at 3498
    Illegal XhoI site found at 8261
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3653
    Illegal NgoMIV site found at 7321
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1543
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 2003
    Illegal AgeI site found at 4706
    Illegal AgeI site found at 5067
    Illegal AgeI site found at 6644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1546
    Illegal BsaI site found at 2183
    Illegal BsaI site found at 5247
    Illegal BsaI site found at 5784
    Illegal BsaI site found at 6647
    Illegal BsaI site found at 7470
    Illegal BsaI.rc site found at 401
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 6641
    Illegal BsaI.rc site found at 7464
    Illegal BsaI.rc site found at 7734
    Illegal BsaI.rc site found at 8896
    Illegal BsaI.rc site found at 9280
    Illegal SapI.rc site found at 1908
    Illegal SapI.rc site found at 4972


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Categories
Parameters
None