Part:BBa_K239001
Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70
Sequence contains 2 phosphorylated BaeR (Bacterial Adaptative rEsponse) binding sites, 1 phosphorylated CpxR (Conjugative Plasmid eXpression) binding site and transcription is initiated by sigma factor 70. Experiments by Bury-Moné et al. (2009) indicates that also RcsB (Regulator of colanic acid Capsule Synthesis) induces expression of the Spy gene.
Function: Can detect various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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