File:BBa K4214003 SDSPAGE.png
Revision as of 13:21, 9 October 2022 by Zeniazen5 (Talk | contribs) (A production culture was set up using BL21 ''E. coli'' and induced with 0.5 mM IPTG once their ODs reached 0.5. The cultures were left overnight at 30°C for protein production. The cells were then harvested, pelleted and purified using a HisPur Cobalt...)
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A production culture was set up using BL21 E. coli and induced with 0.5 mM IPTG once their ODs reached 0.5. The cultures were left overnight at 30°C for protein production. The cells were then harvested, pelleted and purified using a HisPur Cobalt resin. The SDS-PAGE analysis of the production process is shown on the right.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 13:21, 9 October 2022 | | 1,080 × 720 (284 KB) | Zeniazen5 (Talk | contribs) | A production culture was set up using BL21 ''E. coli'' and induced with 0.5 mM IPTG once their ODs reached 0.5. The cultures were left overnight at 30°C for protein production. The cells were then harvested, pelleted and purified using a HisPur Cobalt... |
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