Part:BBa_K4162118
ribozyme+RBS+CDS module: crtEBIY
Introduction
This biobrick was created through standard biobrick assembly of K4162010(ribozyme+T7_RBS+crtE), K4162013(ribozyme+T7_RBS+crtB), K4162016(ribozyme+T7_RBS+crtI) and K4162019(ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, crtEBIY are separated by hammerhead ribozyme, which can perform self-cleaving. Therefore, the long piece of mRNA it transcribes into will be spontaneously separated into four segments, which correspond to crtE, B, I and Y repectively. The translation process of these four enzymes is independent of each other, and the proportion of the expression products of four enzymes can be accurately adjusted by monitoring the intensity of RBS.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2202
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 1811
Illegal AgeI site found at 839 - 1000COMPATIBLE WITH RFC[1000]
| None |