Coding

Part:BBa_K4343108

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 13:07, 9 October 2022 by YueShu (Talk | contribs)

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MaElo2

MaElo2 encodes C16/18 elongase from Mortierella alpina which functions in the desaturase and elongase pathway and could convert C16:0 to C18:0.

Description

The wild-type Y. lipolytica Po1f can produce C18:0 under the catalysis of C16/18 elongase. MaElo2 encodes C16/18 elongase of 266 amino acid from Mortierella alpina. Heterologous expression of MaElo2 could catalyze C16:0 to C18:0 in Y. lipolytica.

The enzyme has catalytic activity for C18:1, and according to the experimental results, it produces almost double the percentage of C18:2.

Reference

Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 845


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Parameters
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