Translational_Unit

Part:BBa_K216004

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-09-25)
Revision as of 10:19, 25 September 2009 by Registry (Talk | contribs)

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Trz hybrid signal transduction protein

Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163. This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/receptor
Parameters
None