Part:BBa_K4414001

nuclear localization signal（NLS）

This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.

Usage and Biology

A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus.(Cokol et al., 2000)

Sequecing

The plasmid was sequenced correct.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional characterization

We can connect NLS to EGFP and observe the location of fluorescent proteins to verify the function of NLS.

Reference

[1].Weikum ER, Knuesel MT, Ortlund EA, Yamamoto KR. Glucocorticoid receptor control of transcription: precision and plasticity via allostery. Nat Rev Mol Cell Biol. 2017 Mar;18(3):159-174. doi: 10.1038/nrm.2016.152. Epub 2017 Jan 5. PMID: 28053348; PMCID: PMC6257982.