Generator

Part:BBa_K274220

Designed by: Siming Ma   Group: iGEM09_Cambridge   (2009-09-19)
Revision as of 02:23, 25 September 2009 by Msmsimon (Talk | contribs)

CrtEBIY under pBad promoter

This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500.

Enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 converts colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene).

Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone).

Datasheet for Part BBa_K274200, Part BBa_274210 and Part BBa_274220 in E. coli strain MG1655. You may also wish to refer to the "Experience" page.

Carotene datasheet1.JPG Carotene datasheet2.JPG

For PDF version of this Datasheet: File:Carotene.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2728
    Illegal NgoMIV site found at 2858
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1943
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None