Tag

Part:BBa_K4156096

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 08:46, 9 October 2022 by Hz (Talk | contribs)

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SsrA tag (ANH)

A metabolically stable tmRNA in bacteria.


Usage and Biology

It has a unique structure with an alanyl tRNA-like structure at its 5′ and 3′ terminal sequences and an intermediate sequence encoding a tag peptide (tag peptide). In line with its unique structure, tmRNA has both tRNA and mRNA functions and participates in a specific translation reaction in cells, trans-translation, resulting in a chimeric protein with a tag peptide attached to the C-terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None