DNA

Part:BBa_K4156122

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 08:43, 9 October 2022 by Hz (Talk | contribs)

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attP Bxb1-Forward

attP Bxb1-Forward is is a recombination sites and it is forward attP sequence, which is recognized by serine integrase Bxb1.

Usage and Biology

The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. We introduce this element to control the switching of genetic logic gates.So this sites can control the amplified gene switch.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27


[edit]
Categories
Parameters
None