Coding

Part:BBa_K4156088

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-06)
Revision as of 08:38, 9 October 2022 by Hz (Talk | contribs)


Integrase Bxb1

Bxb1 integrase serves as a highly efficient DNA recombinase


Usage and Biology

The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. We introduce this element to control the switching of genetic logic gates It also acts as a genetic signal amplifier.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


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Categories
Parameters
None