Signalling

Part:BBa_K4275000

Designed by: Danni Huo   Group: iGEM22_GreatBay_SCIE   (2022-09-29)
Revision as of 08:16, 9 October 2022 by Katie-Wei (Talk | contribs)


Kluyveromyces marxianus alpha mating-factor secretion signal 

K.marx mα is a α-mating secretion signal in Kluyveromyces marxianus that encodes for a α-mating factor (αMF) domain fusing with desired protein. The signal peptide in αMF domain expressed direct the fusion protein into endoplasmic reticulum (ER), Golgi body and thus secrete in vitro. 
 K.marx αMF is integrated upstream of gene of interest (e.g FAST-PETase) to express a αMF domain fused with desired protein (enzymes e.g PETase), which direct the enzyme to secrete from the host cell. The design eliminates process of lysing host cell and purifying desired proteins therefore reduce the cost of the whole textile degradation process. 
 The integrated part αMF also provides a inspiration of extracellular protein secretion for future iGEM teams, paving the way of improving the efficiency of their protein secretion system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None