Part:BBa_K4343097

EgDes5

EgDes5 encodes ∆-5 desaturase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C20:3 to C20:4.

Description

The EgDes5 gene encodes a ∆-5 desaturase of 449 amino acids from Euglena gracilis that catalyzes the conversion of dihomo-γ-linolenic acid (DGLA; C20: 3 n-6) to arachidonic acid (ARA; C20:4 n-6).

References

    1.	Hu, M.; Zhang, C.; Mu, Y.; Shen, Q.; Feng, Y., Indole affects biofilm formation in bacteria. Indian J Microbiol 2010, 50 (4), 362-8.

Pollak, D.W. et al. Isolation of a ∆5 desaturase gene from Euglena gracilis and functional dissection of its HPGG and HDASH motifs. Lipids 47, 913–926 (2012).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 42
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 963
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 1367