Part:BBa_K4192120

Plac-obcA-obcB-Fpoar, Oxalate secretion gene circuit

The expression products of obcA and obcB are enzymes needed to produce oxalate from citric acid and acetyl-CoA. And FpOAR protein can efflux oxalate. The composite part of these three genes can efflux oxalic acid out of the thallus. Because it only have one promoter at the beginning of the whole genetic circuit, the efflux rate of the generated oxalic acid is relatively low, which may also lead to the death of the bacteria more easily due to excessive accumulation of oxalic acid in the body. We have already tested it in E.coli and Pseudomonas fluorescens.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2552
  Illegal PstI site found at 397
  Illegal PstI site found at 2695
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2552
  Illegal PstI site found at 397
  Illegal PstI site found at 2695
  Illegal NotI site found at 2330
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2552
  Illegal BglII site found at 1541
  Illegal BglII site found at 3555
  Illegal XhoI site found at 304
  Illegal XhoI site found at 1428
  Illegal XhoI site found at 3367
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2552
  Illegal PstI site found at 397
  Illegal PstI site found at 2695
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2552
  Illegal PstI site found at 397
  Illegal PstI site found at 2695
  Illegal NgoMIV site found at 389
  Illegal NgoMIV site found at 720
  Illegal NgoMIV site found at 748
  Illegal NgoMIV site found at 784
  Illegal NgoMIV site found at 1014
  Illegal NgoMIV site found at 1791
  Illegal NgoMIV site found at 2208
  Illegal AgeI site found at 326
  
• 1000
  COMPATIBLE WITH RFC[1000]