Composite

Part:BBa_K4156110

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 06:46, 9 October 2022 by Liwei (Talk | contribs)


pPepT-mRFP

pPepT-mRFP is a biological brick that expresses mRFP controlled by the pPepT promoter (BBa_K4156110). pPepT-mRFP functions to characterize the performance of the pPepT promoter using a red fluorescent signal.


Usage and Biology

In this biobrick, we used the pPepT promoter to regulate the expression of downstream mRFP and eventually introduced the rrnB T1 and T7Te dual terminators. In addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pPepT promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pPepT promoter to changes in oxygen concentration.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 723
    Illegal AgeI site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None