Composite

Part:BBa_K4137008

Designed by: YA-JHIH KO   Group: iGEM22_GEMS_Taiwan   (2022-08-20)
Revision as of 06:40, 9 October 2022 by Andreahuang (Talk | contribs)


mleR expressing construct

This construct produces and purifies mleR, the malate-induced regulator that binds to p_mleS promoter for the production of ccdA antitoxin.

Fig.1 Malate-binding transcriptional activator + 6x His-Tag complete construct.

Construct Designs

We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS (AAGGAG) are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None