Composite

Part:BBa_K4463002:Design

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)
Revision as of 06:07, 9 October 2022 by Qiaowa (Talk | contribs) (→‎Design Notes)

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SUMO-CsgA-SmtA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 863
    Illegal AgeI site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The attachment of SmtA to CsgA, which is the major curli subunit protein of E.coli, allows SmtA to form MT proteins in the outer membrane of e.coli, thus achieving the adsorption of metal ions in waste water. SUMO tag was added to improve the expression and solubility of CsgA-SmtA recombinant protein.

Source

SUMO is cloned from PET-28a plasmid, CsgA is from E.coli and SmtA is from Synechococcus sp. PCC7942.

References