Composite

Part:BBa_K4156100

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 05:55, 9 October 2022 by Liwei (Talk | contribs)


pCadC-TP901-φ174E

pCadC-TP901-φ174E is a composite part that express lysis gene φ174E, constructed with pH-sensitive promoter pCadC and serine integrase TP901.


Usage and Biology

We designed pCadC-TP901-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pCadC promoter and TP901. We will validate the function of this biobrick by measuring the viability of the bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1632
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None