Regulatory
Part:BBa_K4343124:Sequence, Features, and Subparts
Designed by: Xiaolu Sun Group: iGEM22_NNU-China (2022-10-09)
Revision as of 05:41, 9 October 2022 by Milulu (Talk | contribs) (Created page with " __NOTOC__ <partinfo>BBa_K4343124 short</partinfo> Derived from Y. lipolytica, YATin promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can...")
YAT
Derived from Y. lipolytica, YATin promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. This promoter has been used to control the expression of the required enzyme genes in Y. lipolytica to iprove the production of omage-3 polyunsaturated fatty acids.
Reference: Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 604
Illegal NheI site found at 959 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 828
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1153