Regulatory
Part:BBa_K4343122:Sequence, Features, and Subparts
Designed by: Xiaolu Sun Group: iGEM22_NNU-China (2022-10-09)
Revision as of 05:35, 9 October 2022 by Milulu (Talk | contribs) (Created page with " __NOTOC__ <partinfo>BBa_K4343122 short</partinfo> Derived from Y. lipolytica, TEF promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be...")
TEF
Derived from Y. lipolytica, TEF promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP.
Some studies indicate that endoglucanase II was successfully expressed in Yarrowia lipolytica under the control of the TEF promoters.
Reference: Boonvitthya, N., Bozonnet, S., Burapatana, V., O’Donohue, M. J., & Chulalaksananukul, W. (2013). Comparison of the heterologous expression of Trichoderma reesei endoglucanase II and cellobiohydrolase II in the yeasts Pichia pastoris and Yarrowia lipolytica. Molecular biotechnology, 54(2), 158-169.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 423